Granulocyte collection by continuous-flow filtration leukapheresis.

A safe and simple technique for the ing preformed leukocyte antibodies, as collection of large numbers of granucompared to the median increment of locytes from normal donors, based on 850/cu mm reported following transthe reversible adhesion of granulocytes fusion of leukocytes obtained by difto nylon wool filters, is described. The ferential centrifugation. The safety and procedure is well tolerated by donors, effectiveness of these granulocytes for and the granulocytes obtained show the treatment or prevention of bacterial nearly normal bactericidal and phagoinfections in granulocytopenic patients cytic capacity in vitro. However, a surremains to be proven, however our prisingly small median 1-hr posttransinitial experience with such transfufusion increment of 233/cu mm was sions has been encouraging. observed in leukopenic recipients lack-